Fig. 4. Effects of different PKC regulatory domains (RD) on stress-fibre formation. Cells were first transfected with vectors encoding EGFP-RD-PKC, —RD-PKCßII, —RD-PKC, or —RD-PKC and then stimulated with 40 nM LTD₄ for 5 minutes. Following stimulation, the cells were fixed, permeablised, stained for F-actin with Alexa 546 phalloidin and examined by confocal microscopy. Merged images are shown on the right. The results illustrated in this figure are representative of five separate experiments.