Fig. 6. Effects of RhoA and PKC on stress-fibre formation. (A) Cells were transfected with the vector encoding EGFP-N19-RhoA and then stimulated with 100 nM TPA for 15 minutes, and they were subsequently fixed, permeablised, stained for F-actin with Alexa 546 phalloidin and examined by confocal microscopy. (B) Cells were transfected with vector encoding EGFP-RD-PKC- and then stimulated with 300 ng/ml CNF-1 for 16 hours and subsequently processed as in A. (C) Cells were stimulated with 300 ng/ml CNF-1 in the absence or presence of 2 µM GF109203X for 16 hours and then processed as in A. Merged images are shown on the right. The results illustrated in this figure are representative of five separate experiments.