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Fig. 1. Cartoon representation of the MyBP expression constructs used in this study. GFP- and c-myc-tagged MyBP cDNAs were cloned into pEGFP-C1 and pJDp expression vectors and transiently expressed in COS cells. (A) GFP-tagged MyBPs. (B) Myc-tagged mutants of MyBP-C (white). (C) Myc-tagged mutants of MyBP- H (black). The positions of both the GFP epitope and myc at the N-termini are shown. Roman numerals denote the position of each IgI or FNIII-like domain. Each domain contains ~ 100 amino acids.