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Fig. 2. Western blot of GFP-tagged MyBPs expressed in COS cells. Lysates from COS cells transfected with GFP-tagged full-length MyBP-C (GFP/C), the last four domains of MyBP-C (GFP/C7-10) and full-length MyBP-H (GFP/H) were analyzed on a 10% polyacrylamide SDS-PAGE gel, transferred to nitrocellulose for western blotting and probed with an anti-GFP-specific mAb. Mock-transfected COS cell lysate served as a negative control.