Fig. 8. Bar graph demonstrating the co-distribution and cable-forming capacity of various MyBP mutants with MyHC. COS cells were transfected with MyHC and MyBP-C (A) or MyBP-H (B) expression constructs. Two days after transfection the cells were fixed and immunostained with anti-MyHC and anti-c-myc antibody. Doubly transfected cells were then identified by indirect immunofluorescence and scored for the colocalization of MyBP and MyHC and cable formation. The number of cells demonstrating colocalization and/or cable formation is expressed as a percentage of the total number of doubly transfected cells. Data represent average values; bars represent one s.d.; ^** indicates an average difference from full-length MyBP with a P<0.005.