Fig. 4. CAR formation is delayed in the absence of microtubules. Temperature-arrested myo2-gc cdc25-22 cells were released into DMSO (A, filled symbols), 100 µg/ml thiabendazole (B, open symbols); 25 µg/ml MBC (C, yellow filled symbols); or 10 µM latrunculin B. (D, dashed lines). Graphs show the timing of cell cycle events in each case. The appearance of cells containing Myo2 rings (green circles) and septa (black squares) was delayed in both (B) and (C) and no binucleate cells (blue diamonds) were observed in the absence of microtubules. (D) In the absence of actin, Myo2 accumulated at the cell equator and the deposition of septal material occurred but complete septa failed to form.