Fig. 5. TBZ-induced delay in CAR formation is Mad2-dependent. Timing of cell cycle events in myo2-gc cdc25-22 (A,B) or myo2-gc cdc25-22 mad2 (C,D) synchronised by transient temperature shift and release into DMSO (A,C: filled symbols) or 100 µg/ml TBZ (B,D: open symbols). The TBZ-induced delay in the appearance of cells containing Myo2 rings (green circles) and septa (black squares) was substantially reduced in the absence of Mad2, and cell viability showed a corresponding decline (red crosses). As expected, no binucleate cells (blue diamonds) were observed in either strain in the presence of TBZ.