Fig. 10. Effect of anisomycin-mediated JNK re-activation on MHC accumulation. Immunoblots of total lysates from RD cells untreated (C) and treated with anisomycin (AN), with TPA and U0126 in the absence (TPA, U) and in the presence of anisomycin (AN + TPA, AN + U) for 30 minutes, 3 and 5 days. Filters were probed with antibodies recognizing phospho-active JNKs and MHC. The data are representative of three independent experiments.