Fig. 9. Effects of SB203580, U0126 and anisomycin on myosin expression. (A) Immunoblot, using anti-MHC antibody, of total lysates from RD cells, untreated (C) or treated with U0126 in the absence (U) or presence of SB203580 (SB+U), with TPA in the absence (TPA) or in the presence of U0126 (U+TPA), SB203580 (SB+TPA) and anisomycin (AN+TPA) for 6 days. (B) Immunofluorescence microscopy of cells treated as indicated above. The data shown in A and B are representative, respectively, of four and two independent experiments.