Fig. 6. Cutting in orp1-4 is initiated early. cdc10-129 orp1-4 and orp1-4 cells were synchronised by lactose gradient centrifugation in early G2, and then shifted to the restrictive temperature. Septation index is given as a measure of synchrony and was determined by Calcofluor staining (A). Aberrant mitoses (cutting) were detected by DAPI staining (B).