Fig. 4. Translocation to the cell surface of FGF-2 fusion proteins depends on the FGF-2 domain and is compatible with both N- and C-terminal GFP tagging. CHO_FGF-2-GFP, CHO_GFP-FGF-2 and CHO_GFP cells were grown in the presence of doxicyclin for 18 hours at 37°C followed by FACS processing, including antibody treatment, as described in Materials and Methods. (A) Quantitative comparison of GFP-derived fluorescence. (B) Quantitative comparison of PE-derived cell surface fluorescence. For both GFP- and PE-derived fluorescence, the signal produced by CHO_FGF-2-GFP cells was set to 100. The results shown are representative of two independent experiments.