Fig. 8. Identification of FGF-2-GFP-positive microdomains on the extracellular surface of CHO cells. CHO_FGF-2-GFP cells were grown on glass coverslips for 24 hours at 37°C in the presence of doxicyclin. Processing for confocal microscopy was performed as described in the legend of Fig. 6. (A) Merged image of 16 confocal planes spanning the whole depth of the cells. (B) A confocal plane close to the bottom of the cells where they are attached to the glass coverslips.