Fig. 1. Total Has2 mRNA levels in REK clones transfected with Has2 sense and antisense plasmids. RNA from three to four separate RNA isolations from each antisense (A9-A25) and sense (S25-S30) cell line was subjected to RT-PCR analysis. The electrophoretic band densities relative to GAPDH were compared to those with vector only (mock)-transfected cell lines within each experiment. The results are expressed as a percentage of mock controls (±s.e.) for each individual cell line.