Fig. 3. Spreading of the transfected cell lines. (A) Differential interference contrast micrographs of typical mock- (M2) and antisense- (AS22) transfected cells 24 hours after plating. The small lamellipodia in antisense cells are depicted by arrows. (B) Substratum areas occupied by individual cells 6 hours after plating. The data represent means±s.e. of three independent experiments. One-way analysis of variance showed that the cell lines were significantly different (P< 0.01). The cell lines indicated by an asterisk significantly differ from all of the mock lines (Fisher test, P<0.05). (C) The time course of the spreading of the transfected REKs. The areas of individual cells were measured at different time points after plating. The points indicate means±s.e. of 21-96 randomly taken cells for each time and cell line. W, wildtype; M1-M3, mock controls; S25-S30, sense cells; A9-A25, antisense cells.