Fig. 4. Vinculin-positive adhesion plaques and hyaluronan at the cell-substratum interface. (A) Double immunofluorescence for hyaluronan (green) and vinculin (red) in confocal optical sections constituting the undersurface of the keratinocytes. The image of vinculin is processed as for the quantification of adhesion plaques, see Materials and Methods. (B) Quantification of vinculin-positive areas by image analysis in the transfected cell lines. Means and ranges of two separate experiments for each cell line are shown. One-way analysis of variance and Fisher tests were used to indicate the cell lines that were significantly different ^*(P<0.05) from mock controls.