Fig. 5. Proliferation rate of Has2-transfected cell lines. Cell numbers were counted with a hemocytometer at different time points after plating an equal number of cells from each cell line. (A) Has2 antisense cell lines (A22, A23, A25) with wild-type (Wt) and mock-transfection (M2) controls. (B) Has2 sense cells (S27, S29, S30) and the controls. Bars represent the range of duplicate cultures. The antisense lines differed significantly from all other cell types on day 1 (^*P<0.05, analysis of variance).