Fig. 7. Induction of hyaluronan synthesis in the wound edge. Confluent cultures were treated with Streptomyces hyaluronidase to remove existing hyaluronan, scraped with a pipette tip, washed and 8 hours later stained with HABC and FITC-streptavidin for newly synthesized, cell-associated hyaluronan. Examples of areas close to the scraped area (edge) and those more distant (intact) are shown in mock-transfected (M3), antisense (A21) and sense (S29) cell lines. Note the higher hyaluronan signal of the wound edge areas in M3 cultures, a low signal in the A21 cells in both intact and edge areas and a high signal in the S29 cells in both intact and edge areas.