Fig. 2. Expression of Asy1 and BoAsy1 proteins in Arabidopsis and B. oleracea, respectively. (A) Western analysis using the anti-Asy1 antibody of an Arabidopsis bud series from a single inflorescence. Lane 1 represents pooled small (pre-meiotic) buds. Lanes 2 through 12 represent buds of increasing size. Expression is first detected in lane 4 corresponding to early male meiosis. The signal then declines, rising again in lane 10, which corresponds to early female meiosis (late male meiosis). (B) Western analysis using an anti-Asy1 antibody of a meiotically staged anther series and vegetative tissues from B. oleracea. Lanes 1-6 represent interphase, leptotene, zygotene, pachytene, diplotene and tetrad stage, respectively. Lane 7, stem; lane 8, leaf.