Fig. 6. (A) Simultaneous immunolocalization of Asy1 antibody (green) to chromosomal axes and FISH to telomeres (red) of a B. oleracea pachytene PMC. Bar, 10 µm. (B-E) Enlarged termini of individual pachytene bivalents showing that the Asy1 labelling stops short of the telomere repeats. (B) DAPI channel; (C) DAPI + telomere probe; (D) DAPI + Asy1 antibody (E) merged image; note that Asy1 localizes to the chromosome axes but is absent from the surrounding chromatin. Bar, 5 µm.