Fig. 5. Development of oligodendrocytes from ES-cell-derived OPCs. Engineered ES cells were treated and selected as described in Fig. 1. On day 10, the cells were exposed to FGF-2 and Shh for 5 days. Both FGF-2 and Shh were then removed, and the cells were cultured in PDGF-AA and TH for another 9 days (A) or 3 days (B). (A) The cells were stained with anti-GC antibody to identify oligodendrocytes and bisbenzimide to identify cell nuclei. (B) The cultures were stained with anti-neurofilament antibodies to identify neurons (green), with O4 antibody to identify oligodendrocyte lineage cells (red) and with bisbenzimide to identify cell nulei (blue). Bar, 50 µm.