Fig. 1. Degenerate RT-PCR amplification of SNARE cDNAs expressed by CD8 cells. Total RNA from confluent CD8 cells was subjected to reverse transcription followed by PCR amplification using degenerate primers for the coding region of known SNARE isoforms (see Materials and Methods for experimental details). Bands of 266, 370, 379, 244 and 398 bp corresponding to syntaxin-1A, syntaxin-3, syntaxin-4, VAMP-2 and SNAP-23, respectively, were amplified. As a positive control, parallel RT-PCR experiments with total RNA extracted from the rat brain, lung or kidney were performed. The results shown are representative of at least three separate experiments.