Fig. 8. Rap1 activation in response to different stress conditions. After a 1 hour adaptation at 22°C in KK₂ buffer, vegetative Ax-2 cells were subjected to cold shock, heat shock or hypo-osmotic shock, and samples were taken at the time points indicated. Cell lysates were incubated with GST-RalGDS(RBD) as described in the Methods and Materials, and levels of Rap1 bound to GST-RalGDS(RBD) were determined by western blotting.