Fig. 3. The enlarged endocytic vesicles in cells expressing Rab^Q67L are primarily lysosomes. Both wild-type cells (A-F) and RabD^Q67L-expressing cells (G-L) were pulsed with FITC-dextran for 5 minutes and chased for 0 minutes to view macropinosomes (A,B,G,H); chased for 15 minutes to view lysosomes (C,D,I,J); and 60 minutes to view post-lysosomes (E,F,K,L). Cells were gently fixed and viewed using a fluorescence microscope. Bar, 2 µm. The arrows reveal structures described in the text.