Fig. 9. RabD^Q67L promotes fusion of separate phagosomes containing bacteria and beads. Both Ax4 and RabD^Q67L-expressing cells were pulsed with FITC-bacteria for 10 minutes, washed and pulsed with deep blue latex beads (L-1398) for 10 minutes in shaking suspension. After the sequential pulse periods, the cells were washed and allowed to chase in fresh HL-5 while settling on coverslips for 30 minutes. The cells were gently fixed and images were taken of control cells (A,B) and RabD^Q67L cells (C,D) in the GFP and rhodamine channels of a fluorescent microscope. The scale bar represents 3 µm. The red and green arrows reveal separate latex bead and bacterial phagosomes. The yellow arrow marks a phagosome that has beads and bacteria.