Fig. 5. Separate domains of Inh2 are required for cytoplasmic retention and nuclear import. HeLa cells expressing myc-tagged Inh2 proteins were cultured at either low (B,D,F,H,J) or high density (A,C,E,G,I) on fibronectin-coated coverslips. A and B show a phase image for I and J and are representative of all panels. C and D show a near full-length Inh2 [0-197]; E and F are an N-terminally truncated form (residues 78-197); G and H are a C-terminal truncation (residues 0-119) and I and J are of a central construct consisting of residues 78-150. K shows a schematic of the truncations used.