Fig. 7. Inhibitor-2 is actively imported into the nucleus. A and B show low-density HeLa cells expressing either an Inh2GFP₃ fusion (A) or an Inh2GFP₃ fusion in which lysines 142 and 144 have been mutated to alanine, termed NLS mutGFP₃ (B). Cells were visualized with the fluorescence of GFP and the nuclear, cytoplasmic and total fluorescence were quantified. The graph shows the import index for GFP₃, Inh2GFP₃ fusion, NLS MutGFP₃ or Inh2[Thr72Ala]GFP₃ fusion.