Fig. 8. Inhibitor-2 exits the nucleus by passive diffusion. HeLa cells were electroporated with either Inh2GFP₃ or Inh2HA and grown at low density for 6 hours. The cells were then trypsinized and plated onto fibronectin-coated coverslips for 4 hours. The coverslips were then fixed and visualized either for GFP fluorescence or by indirect immunofluorescence techniques directed against the triple HA tag (orange). A shows the GFP fluorescence and B shows a merge of the HA immunofluorescence (orange) and DAPI staining of the DNA (Blue).