Fig. 2. (a) Diagram of rye plant and meiotic development and anther culture methodology. A single rye floret contains three anthers at an equivalent developmental stage. Meiosis occurs synchronously within a single anther and among the three anthers from one floret. The anthers are shown in yellow-green, and meiotic cells are depicted in blue. The meiotic cells cannot be observed through the anther wall; the diagram merely suggests their location. One anther half provides the time 0 control, whereas the halves of a second anther are put into culture. (b) Synchrony of meiosis in rye anthers. A projection of a 3D image of meiotic cells from a single anther. All cells appear to be in metaphase of the first meiotic division, as indicated by the presence of seven bivalents. Bar, 10 µm.