Fig. 3. The effect of colchicine on telomere clustering. To convey the 3D architecture of the cell, the data corresponding to the entire nuclear volume (approximately 100 z-sections) was divided into quarters, and each quarter was projected into a single image (Bass et al., 1997). The resulting images are displayed sequentially. Control and 100 µM colchicine-treated anthers were cultured for 16 hours. Telomeres (green) were detected by FISH using a probe to the telomere repeat, and chromatin (red) was stained with DAPI. Bar, 10 µm.