Fig. 5. The effect of colchicine on bouquet dispersal. Representative nuclei are displayed as sequential projections of the 3D nucleus as in Fig. 3. Control and 100 µM colchicine-treated anthers were cultured for 18 hours. Telomeres (green) were detected by FISH using a probe for the telomere repeat, and chromatin (red) was stained with DAPI. Bar, 10 µm.