Fig. 3. Cytoplasmic MT distribution during early meiotic prophase. To convey the 3D
architecture of the cell, the data corresponding to the entire cellular volume
(approximately 100 z-sections) were divided into quarters, and each quarter
was projected into a single image (Bass et
al., 1997). The resulting images are displayed sequentially. MTs
(green) were detected with an antibody against
-tubulin. Chromatin
(red) was stained with DAPI, and telomere positions were inferred from the
telomeric heterochromatin (a subset indicated with *) in (A) and
(B). (A) Pre-bouquet, (B) bouquet and (C) post-bouquet. Bars, 10 µm.