Fig. 8. Nuclear pore redistribution and polarization in colchicine-treated bouquet-stage cells. (A) Sequential z-axis projections of meiotic nuclei, as described in Fig. 3. Control and 100 µM colchicine-treated anthers were cultured for 12 hours. NPs (green) were immunolocalized using an antibody against nuclear pore complex proteins (mAb414). Chromatin (red) was stained with DAPI. Positions of telomeres were inferred from heterochromatin (a subset indicated with ^*). Bar, 10 µm. (B) NP clustering in colchicine-treated cells. A box-whisker plot of the percentage of the nuclear surface occupied by NPs in control (n=22) and 100 µM colchicine-treated (n=22) nuclei. (C) Polarization of NPs in colchicine-treated cells. A box-whisker plot (as in Fig. 4) of telomere-NP angles (as in Fig. 6) in control (n=30) and 100 µM colchicine-treated (n=34) nuclei, compared with the distribution of random angles in a sphere (n=50). The NP-cell cortex angle is the angle created between the center of the NP-containing region, the center of the nucleus and the center of the cell cortex (Fig. 4B). A diagram of NP-cell cortex angle is shown; the angle is indicated by . A box-whisker plot of NP-cell cortex angle in control (n=8) and 100 µM colchicine-treated (n=12) cells is shown. Also shown is the distribution of random angles in a sphere (n=50).