Fig. 2. Mad1F10 can activate the APC/C in vitro. SDS-PAGE of Xenopus mitotic extracts that were incubated with buffer alone (panel I) or with 20 µM Mad2 (panel II), in the presence of increasing amounts of Mad1F10 (a-e at 0, 10, 20, 40 and 60 µM). Asterisks indicate APC/C activation by Mad1F10.