Fig. 4. Microtubules are required for depletion of Mad2, cytoplasmic dynein, BubR1, and the phosphoepitope 3F3/2 at kinetochores in anaphase in PtK1 cells. (A-D) For all images the top row shows a prometaphase cell, the second row shows a cell induced to enter precocious anaphase by Mad1F10 injection, the third row shows a cell treated with 10 µM nocodazole, and the bottom row shows a cell treated with 10 µM nocodazole and induced to enter anaphase by Mad1F10 injection. Bar, 5 µM.