Fig. 5. Microtubules are required for depletion of INCENP at centromeres in anaphase in PtK1 cells. A prometaphase cell fixed and stained for INCENP (top row). A cell induced to enter precocious anaphase by Mad1F10 injection (second row). A cell treated with 10 µM nocodazole (third row). A cell treated with 10 µM nocodazole and induced to enter precocious anaphase by Mad1F10 injection (bottom row). Bar, 5 µM.