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Fig. 9. Expression of YY-FF (986-987) mutant SHIP2 impairs lamelliodia formation. HeLa cells expressing wild-type SHIP2 (A,B,E,F) or YY-FF (986-987; C,D,G,H) mutant proteins were plated on collagen-I-coated (A-D) or fibronectin-coated (E-H) surface for 60 minutes. Cells were stained with anti-FLAG (M2) antibody to reveal expression of exogenous proteins (green) and counterstained with phalloidin-TRITC (red). Bar, 10 µm.