Fig. 3. HuD, KIF3A and tau mRNA colocalize in RNP granules. (a) Phase image: the growth cone is shown on the right side of the panel. Colocalization of tau mRNA (b, red), HuD protein (c, green) and KIF3A (d, cyan) to yield the merged image presented in e of the axon and growth cone of differentiated P19 cells. The curved arrowhead denotes colocalization of the three components (white). The asterisk denotes colocalization of HuD and KIF (light green). The straight arrowhead denotes colocalization of HuD and tau mRNA (yellow). Bar, 1 µm (x40,000 magnification).