Fig. 4. (A) A field view of differentiated P19 cells treated with KIF3A antisense ODN, analyzed using a confocal micoscope. (a,b) Control untreated cells; (c,d) treated cells; (a,c) GFP-tau fluorescence. (b,d) KIF3A protein localization visualized by immunohistochemical staining with KIF3A antibodies. Bar, 20 µm. (B) Immunoblot analysis of cell extracts prepared from control-differentiated P19 cells or from cells treated with KIF3A antisense ODN and KIF3A sense ODN. The blots were reacted with KIF3A, KIF1A, tau, tubulin, MAP2, synaptophysin and neurofilament antibodies. (This is a representative blot from three experiments.)