Fig. 5. Activation of a single channel during osteoclast spreading. Spontaneous electrophysiological activity was studied at single channel level during osteoclast membrane spreading. (a) For cell-attached recording, the pipette tip was located in the cell nuclei area (nuclei are represented by dotted circles). (b) Burst openings of a single channel with a unitary conductance of 22 pS (transmembrane potential=-80 mV). (c) Time evolution of the open probability for the observed channel. The open probability peaks (dotted box) correspond to the channel burst openings.