Fig. 8. Correlation analysis between membrane spreading kinetics and whole-cell current. (a) A polar diagram of the cell perimeter during spreading; the red and blue polygons correspond to the initial and final states of the cell, respectively. (b) A polar representation of the correlation coefficient between membrane current and each axial rate of membrane spreading. The correlation was higher for axes where the movements' amplitude was larger and lower for zones with slight moves or retractile displacements. (c) The red line represents the global spreading rate calculated with multiple regression analysis and the black line the membrane current. This shows a very significant correlation between multiple regression of spreading rates and IK_Ca current. (d) Coherence analysis between membrane current and membrane spreading rate; the peaks indicate the presence of frequencies for which both phenomena are in phase.