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Fig. 5. GFP secretion is independent of factors present in serum. CHO cells transiently expressing GFP were starved, labeled with [35S]-methionine and chased for 6 hours in the presence of various concentrations of serum as indicated. The cell lysates (C) and media (M) were harvested and immunoprecipitated with anti-GFP antibodies. `% Sec.' denotes the percentage of secretion.