Fig. 8. Two different forms of GFP are present in CHO cells but only the improperly folded form is secreted. CHO cells expressing GFP (CHO+GFP) were incubated at 37°C in the presence of 100 µM cycloheximide for 0 or 6 hours. (A) The cell lysate (C) and media (M) were harvested at the end of the incubation, immunoprecipitated and immunoblotted with anti-GFP antibodies. (B) Spectro-fluorometric measurements of 200 µl aliquots of the cell lysate and media harvested as above are plotted as RFU per ml. Each bar on the graph represents the average value derived from five independent samples.