Fig. 1. Maturation of vacuolar enzymes ALP and CPY. Wild-type (ARF1/GCS1) and gcs1 and arf1 mutant cells were grown, radiolabeled with ³⁵S-labeled methionine and cysteine and incubated for the indicated chase time (min) before immunoprecipitation. p1 CPY is the core-glycosylated form found in the ER, p2 is the outer chain-glycosylated Golgi form and the mature form (M) results from proteolytic processing in the vacuole. pALP is the proenzyme form and mALP is the mature form in the vacuole.