JCS -- Huang et al. 115 (2): 275 Figure IG2

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Fig. 2. Detection of Gcs1p and mutated forms in yeast by western blot analysis. The indicated amounts of proteins from (A) YPH 252 wild-type or gcs1 mutant cells and (B) purified His-tagged Gcs1p or (C) $~$ 20 µg of proteins from gcs1 mutant cells overexpressing wild-type Gcs1p, HA-tagged zinc-finger-mutated ( ${Delta}$ Zn) Gcs1p, PH domain-deleted ( ${Delta}$ PH) Gcs1p or GFP-fused PH domain (GFP-PH) were separated by SDS-PAGE. Proteins were stained with Coomassie blue (A) or transferred to PVDF, reacted with affinity-purified antibodies against Gcs1p and detected using the ECL system. Positions of protein standards (kDa) are at the left.