Fig. 6. Effect of overexpression of Gcs1p on mitochondrial morphology does not require its zinc-finger motif. Data are presented as in Fig. 3. Upper panel, lysates of spheroplasts from gcs1 cells overexpressing Gcs1Zn and grown in minimal medium were fractionated for analysis by immunoblotting. Lower panel, indirect immunofluorescence staining of gcs1 cells overexpressing Gcs1Zn. Spheroplasts were reacted with purified anti-Gcs1p antibody (B) or anti-porin antibody (C) or stained with H33258 (A). Arrows indicate tubular mitochondria and arrowheads mitochondria.