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Fig. 7. The C-terminal PH domain of Gcs1p is required for mitochondrial localization. Data are presented as in Fig. 3. Upper panel, lysates of spheroplasts from gcs1 cells overexpressing Gcs1{Delta}PH and grown in minimal medium were fractionated. Lower panel, indirect immunofluorescence staining of spheroplasts reacted with purified anti-Gcs1p antibody (B) or anti-porin antibody (C) or stained with H33258 (A).