Fig. 2. GFP-SH2 is only recruited by autophosphorylation-competent EGF receptors. GFP-SH2-transfected NR6 fibroblasts expressing either the c’1000 EGF receptor truncation mutant (NR6 c’1000; A,B) or the c’1000 EGF receptor with the mutation Y992F (NR6 c’1000F; C,D) were observed in parallel. The cells were either untreated (A,C) or stimulated with EGF-TR for 20 minutes (B,D). Cells were visualized by confocal fluorescence microscopy.