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Fig. 5. The segregation of active EGF receptors from accessible PtdIns(4,5)P2 is not specific to NR6 fibroblasts. A431 cells transfected with GFP-SH2 (A,B) or GFP-PH (C,D) were either untreated (A,C) or stimulated with EGF-TR for 20 minutes (B,D). Cells were visualized by confocal fluorescence microscopy.