Fig. 3. Cell-cycle profiles showing cells expressing wild-type and Del236-241 EGFP-emerin at the same stage in the cell cycle. The PI area (DNA content) and EGFP intensity were both monitored so that cell-cycle profiles show the relative number of cells in G1, S or G2M for each population (untransfected and transfected). In each profile, the orange lines represent the untransfected populations and the black, purple and blue lines represent cells expressing EGFP-emerin 28, 32 and 35 hours after being released from G2M. Cells expressing wild-type EGFP-emerin 28 hours after synchronisation were in G1-S, whereas cells expressing Del236-241 EGFP-emerin 35 hours after synchronisation were in G1-S. Thus cells expressing Del236-241 EGFP-emerin lag behind those cells expressing wild-type EGFP-emerin by six to seven hours per cycle.