Fig. 10. Primary muscle fibroblasts at passage 2 (A-C) and passage 4 (D-E) grown in HS-2+Gal-M for 2-3 days. Cells are negative for desmin in both the absence (A) and presence of GAL-M (D). Cells are positive for vimentin (B) and negative for myosin II heavy-chain in the absence (C) and presence (E) of GAL-M. Nuclei are stained with DAPI. Bar, 50 µm.